Italian Guidelines for the diagnosis and treatment of Fetal Alcohol Spectrum Disorders: international diagnostic criteria - differences and similarities

Ginevra Micangeli1, roberto paparella1, Michela Menghi1, Mauro Ceccanti2, Giovanna Coriale3, DANIELA FIORENTINO4, Harold Eugene Hoyme5, Marco Fiore6, GIAMPIERO FERRAGUTI7, Giovanni Corsello8, SIMONA PICHINI9, Luigi Tarani1; Interdisciplinary Study Groups* SAPIENZA, ISTAT, ISS, AIDEFAD, SITAC, SIFASD, FIMMG-Lazio, SIPPS, SIMPeSV, CIPe

1Department of Maternal Infantile and Urological Sciences, Sapienza University of Rome, Italy; 2SITAC - Società Italiana per il Trattamento dell’Alcolismo e le sue Complicanze, Rome, Italy; 3CRARL Lazio, ASL Roma 1, Rome, Italy; 4ASL Rieti, Italy; 5Sanford Children’s Genomic Medicine Consortium, Sanford Health; Department of Pediatrics Sanford School of Medicine, University of South Dakota, Sioux Falls, South Dakota, USA; 6Institute of Biochemistry and Cell Biology (IBBC-CNR), Department of Sensory Organs, Sapienza University of Rome, Italy; 7Department of Experimental Medicine, Sapienza University of Rome, Italy; 8Department of Sciences for Health Promotion and Mother and Child Care “G. D’Alessandro”, University of Palermo, Italy; 9National Centre on Addiction and Doping, Istituto Superiore di Sanità, Rome, Italy.

Equally contributed as the first authors.

Summary. The umbrella term Fetal Alcohol Spectrum Disorders (FASD) brings together under its definition a heterogeneous continuum of disabilities linked by a common etiology and pathogenesis: exposure to alcohol during intrauterine life. Despite extensive research, definitive toxic thresholds remain elusive, underscoring the recommendation for complete alcohol abstinence during pregnancy and lactation. FASD poses diagnostic challenges due to its varied presentations and heterogeneous phenotype. Consequently, no singular diagnostic guideline exists, with multiple expert-driven diagnostic systems globally available. This review aims to synthesize recent and notable guidelines facilitating FASD diagnosis. While efforts were made to include the latest diagnostic systems, determining which scheme is best applied to each individual patient population necessitates clinician discretion. In Italy, the guidelines proposed by Hoyme, revised in 2016, are commonly utilized, yet comparative analysis among guidelines offers valuable insights into their historical context and diagnostic utility. Our discussion explores both similarities and discrepancies among systems for diagnosing FASD, shedding light on their evolution and practical application. The objective of our work was to compare in a practical and precise manner the various existing guidelines used globally regarding the diagnosis of FAS. Our review therefore proposes the diagnostic criteria used by the various working groups and compares them, trying to create a practical comparison between the various guidelines, identifying differences and similarities.

Key words. Alcohol, children, diagnostic criteria, FASD, guidelines, pediatrician.

Linee guida italiane per la diagnosi e il trattamento dei disturbi dello spettro feto-alcolico: criteri diagnostici internazionali: differenze e somiglianze.

Riassunto. Il disturbo dello spettro feto-alcolico (FASD) riunisce sotto la sua definizione un quadro eterogeneo di patologie accomunate dalla comune eziopatogenesi: l’esposizione fetale all’alcol. Nonostante ricerche approfondite, la soglia tossica non è stata ancora definita, obbligando alla raccomandazione di una completa astinenza dall’alcol durante la gravidanza e l’allattamento. La FASD pone anche sfide diagnostiche a causa della sua presentazione clinica varia e del fenotipo eterogeneo. Di conseguenza, non esiste una singola linea guida diagnostica, ma molteplici linee guida, disponibili a livello globale, redatte in base alle competenze prevalenti degli estensori. Questa revisione mira a sintetizzare le linee guida più utilizzate che consentono la diagnosi di FASD. Sebbene siano stati compiuti notevoli sforzi per sintetizzare le linee guida più recenti, non ne esiste ancora una che racchiuda tutti i criteri contenuti nelle precedenti, pertanto la diagnosi contiene ancora elementi di discrezionalità del medico che la pone. In Italia, le linee guida proposte da Hoyme et al., riviste nel 2016, sono comunemente utilizzate, ma l’analisi comparativa tra le linee guida offre preziosi spunti sul loro contesto storico e sull’utilità diagnostica, ponendo le basi per un’ulteriore proposta che le riassuma tutte. Questo lavoro esplora sia le somiglianze che le differenze tra le varie linee guida esistenti, facendo luce sulla loro evoluzione e applicazione pratica.

Parole chiave. Alcol, bambini, criteri diagnostici, FASD, linee guida, pediatra.

Introduction

The umbrella term fetal alcohol spectrum disorders (FASD) is used to describe the range of possible physical, behavioral and neurodevelopmental effects in individuals with prenatal alcohol exposure (PAE)1. In a meta-analysis of 24 studies, which used active case ascertainment or clinic-based methods specifying the diagnostic guideline or case definition adopted, the global prevalence of FASD among children and youth in the general population was estimated to be 7.7 per 1000, and 1 of 13 pregnant women who consumed alcohol during pregnancy was estimated to deliver a child with FASD2,3.

Alcohol is a teratogen with significant adverse effects on central nervous system (CNS) development4-10. During pregnancy, a safe threshold of amount or time of alcohol consumption has not been established; it is therefore recommended that women completely avoid drinking alcohol while pregnant or trying to conceive, since FASD is the most frequent, totally avoidable and preventable cause of acquired intellectual disability in childhood4,11-14.

There are no unique physical or neurodevelopmental features that are individually specific or sensitive enough to confirm FASD diagnoses, nor is there a diagnostic laboratory test. Several diagnostic schemes exist. However, despite some shared characteristics, they differ in terminology, sensitivity and specificity, leading to confusion and lower confidence in obtaining a valid diagnosis15,16.

Here, we briefly review the nomenclature and criteria used by the various diagnostic systems adopted internationally. The main differences usually involve the number of sentinel facial features required to diagnose FASD, cutoffs for inclusion of any specific clinical feature, the inclusion or exclusion of growth deficit as a diagnostic criterion, the type of neurodevelopmental evaluation employed in the diagnostic approach, and the methods to confirm PAE17,18. The diagnostic systems discussed in this paper are set forth in table 1.




Historical overview of FASD diagnosis

Despite ancient references to the harmful effects of PAE, the associated physical malformations and neurodevelopmental disabilities were first described in the medical literature in 196813,19. In France, after a 1957 thesis dissertation by Jacqueline Rouquette (describing what was later termed FAS)20, Paul Lemoine et al. reported on 127 cases of children born to alcoholic women with a singular pattern of morphological and cognitive characteristics21. Subsequently, in the USA, in 1973 Jones and Smith analyzed the recurrent pattern of structural alterations in the children of drinking women and coined the term “Fetal Alcohol Syndrome (FAS)”18,22-25. More recently, given the wide variety and degree of severity with which the teratogenic effects of PAE can present, and as pediatricians became more familiar with the clinical presentation of PAE, experts in the field advocated for assignment of distinct PAE-related diagnostic categories within a continuum (FASD), from mild to severe2.

International guidelines and variations

Institute of Medicine Criteria

In 1996, the US Institute of Medicine (IOM) published seminal guidelines for the identification and classification of children prenatally exposed to alcohol. The IOM broadly set forth four distinct diagnostic categories within FASD: FAS (with or without confirmed maternal alcohol exposure), partial fetal alcohol syndrome (PFAS), alcohol-related neurodevelopmental disorder (ARND), and alcohol-related birth defects (ARBD). However, the 1996 IOM report did not offer practical guidance to clinicians about how to assign children with PAE to the diagnostic categories. In 2005, Hoyme et al. operationalized the IOM classification by describing specific clinical recommendations to be used to assign diagnoses within the 1996 IOM rubric26-29.

In 2016, as part of an NIAAA sponsored effort to define the prevalence of FASD in the US (CoFASP, the Collaboration on Fetal Alcohol Spectrum Disorders Prevalence), updated guidelines were published, clarifying and expanding the original Hoyme 2005 classification. In particular, the authors set forth the following: precise definitions for documented PAE; neurobehavioral criteria for FAS, PFAS, and ARND diagnoses; revised diagnostic criteria for ARBD; an updated the dysmorphology scoring system; and a new lip/philtrum guide for the white population28,30.

The evaluation of maternal prenatal alcohol consumption constitutes an essential component of the diagnostic process, serving as the initial stage in evaluating children with reported PAE31. As mentioned previously, a consensus definition of significant PAE was defined by Hoyme et al in 2016; this is fundamental, since affirmative validation of alcohol exposure is imperative for the assignment of a diagnosis of ARND or ARBD; whereas, according to the Hoyme criteria, certain situations allow for the diagnosis of FAS or PFAS in the absence of definitive documentation of prenatal alcohol use5,19,24.

According to the CoFASP guidelines, once alcohol exposure has been established, characteristic structural features of FASD must subsequently be assessed. Growth deficiency (with respect to stature and/or weight) and small head circumference are defined as ≤10th centile. A complete dysmorphology examination follows, with criteria for sentinel facial features being met if at least two of the three specific facial characteristics of FASD are present: short palpebral fissures (≤10th centile), smooth philtrum and thin vermilion border of the upper lip (rank 4 or 5 on a racially normed lip/philtrum guide, if available)26,28,32-35.

The initial 2005 Hoyme guidelines permitted the assignment of FASD diagnoses in children exhibiting the requisite facial features, growth restriction, and/or microcephaly, even in the absence of substantial neurobehavioral impairment14,25,36. The updated 2016 guidelines mandate that all children designated with FASD diagnoses, excluding those with ARBD, must display neurobehavioral impairment, specifically defined as cognitive impairment or behavioral impairment without concurrent cognitive deficits (cutoffs for neuropsychological testing are <–1.5 SD). For children aged ≤3 years, a diagnosis of FAS and PFAS may be established when there is observable evidence of developmental delay <1.5 SD below the mean; however, a conclusive diagnosis of ARND cannot be assigned before the age of 3 years37.

ARBD diagnosis requires both documented PAE and ≥1 specific major malformation (cardiac, skeletal, renal, ocular, and ear-related) demonstrated in animal models and human studies to be the result of PAE3,38,39. The approach by Hoyme et al recommends assignment of an FASD diagnosis only after considering genetic disorders or conditions stemming from prenatal exposure to other teratogens among possible differential diagnoses15,40.

The authors assert that the assessment of individuals prenatally exposed to alcohol requires a multidisciplinary team approach with medical assessment and team leadership by a pediatrician or clinical geneticist with expertise in the full range of human malformation syndromes and the dysmorphology evaluation of children with FASD. In addition, exposed children should have expert psychological/neuropsychological assessment, and a skilled interviewer should evaluate prenatal maternal alcohol intake. Other team members may include developmental behavioral pediatricians, psychiatrists, speech pathologists, occupational therapists, physical therapists, special educators, audiologists, and/or ophthalmologists, among others12,14,17.

The ideal team members, in part, will be determined by the age of the individual being assessed (infant, child, adolescent or adult). In small or isolated communities where essential team members are not available, the community team must collaborate and consult with larger centers that have a more comprehensive diagnostic team. Access to diagnosis can also be enhanced by telemedicine41,42. Unfortunately, MDTs are not universally available, and children at-risk often present to primary care providers who may not be sufficiently educated in the diagnosis of FASD. Thus, education of primary care providers in this field is an urgent priority17. It should be noted that the Hoyme et al diagnostic criteria are the most extensively used in international FASD prevalence studies.

4-digit diagnostic code (2004, 3rd edition)

The 4-digit code is another diagnostic tool for diagnosing the full spectrum of outcomes observed among individuals with prenatal alcohol exposure43,44. The first edition of the FASD 4-digit diagnostic code was developed by the Washington State FAS Diagnostic and Prevention Network (FAS DPN) in 1997 and published in peer-reviewed form in 200045,46. The 4-digit diagnostic code allows clinicians to make diagnoses by assigning Likert-scaled numerical ratings for four key clinical parameters assessed in the evaluation of a potential FASD. The method uses case-specific and non-operator-dependent definitions3,34,47. The four digits of the code reflect the variability of the four key diagnostic features of FASD. The following terms are used in various combinations to name the 22 diagnostic categories:

1. Growth deficit;

2. Morphological facial characteristics of FAS;

3. Central nervous system (CNS) abnormalities;

4. Prenatal exposure to alcohol.

The expression of each characteristic is classified independently from the others on a 4-point Likert scale where 1 reflects the complete absence of the anomaly connected to FASD and 4 coincides with the presence of a pathological trait typical of FASD28,44. There are 256 possible 4-digit diagnostic codes ranging from 1111 to 4444 based on the combination of the various clinical characteristics observed in the patient45,48,49. Each 4-digit diagnostic code falls into one of 22 unique clinical diagnostic categories (labeled A through V).

The 4-Digit Code also documents and classifies all other prenatal and postnatal risk factors that often accompany prenatal alcohol exposure. The authors suggest that this is a diagnostic methodology valid for all age groups, from birth to adulthood13,25.

Like the Hoyme criteria, the 4-digit code is designed to be used in a multidisciplinary team setting (which may include a physician, a psychologist, an occupational therapist, a speech pathologist, a social worker, a family advocate and a public health professional, among others)50,51.

The names assigned to each diagnostic category reflect the patient’s clinical outcome and alcohol exposure. The first three categories (A through C) meet the criteria for a clinical diagnosis of FAS and are named as such. The fourth category (D) applies to the patient who presents with all of the features of FAS but has a confirmed absence of prenatal alcohol exposure from conception to birth. This category is referred to as an FAS Phenocopy. The remaining 19 categories (E through V) do not meet the minimum criteria for FAS or partial FAS. These are subsequently named to reflect the Likert ranking of each digit in the 4-digit Diagnostic Code. Many of these patients might have previously been referred to variably as having PFAE, ARBD, or ARND15,44,52. Diagnostic categories E-I would have previously been referred to as ‘fetal alcohol effects’, ‘alcohol-related birth defects’, or ‘alcohol-related neurobehavioral disorder’. Categories J–V are new categories that describe a large number of patient groups that have never been adequately classified or described in the past. The Likert ranks for the four digits of the code are case-defined for consistent application1,2,53. 

The 4-Digit Diagnostic Code offers another objective evidence-based approach to reporting outcomes and exposure that reflects the diversity of disability associated with prenatal alcohol exposure. Preliminary assessments of precision, accuracy and power appear to be increased over the ‘gestalt’ method of diagnosis54-56. This can be attributed, in large part, to the use of objective continuous measurement scales, specific, comprehensive case definitions and the use of a multidisciplinary clinical team approach57-59.

The 4-Digit Diagnostic Code is fully comprehensive; similar to the Hoyme criteria, it can be used to diagnose individuals of all ages and races who present across the full spectrum of exposure and outcomes. This is achieved by directing the clinician to age-, gender-, and ethnically-adjusted anthropometric and psychometric measures when available and appropriate16,51,60. This method can be taught to a wide array of healthcare and social service providers and has been adopted in many multidisciplinary FASD diagnostic clinics in the US and Canada61,62.

The 4-Digit Diagnostic Code has been criticized as being excessively difficult to deploy in a clinical setting26. It differs from the 2005 and 2016 Hoyme criteria by requiring all three sentinel facial features to be present to make a diagnosis of FAS (as opposed to two of three in the Hoyme guidelines). In addition, cut-offs for growth deficiency and microcephaly are set at the 3rd centile in the 4-digit code as opposed to the 10th centile in the Hoyme guidelines. Both require accurate assessment of growth, development and prenatal alcohol exposure. The Hoyme guidelines are therefore likely to be more sensitive but less specific than the 4-digit code.

Centers for Disease Control and Prevention (2004)

In 2002, the US Congress mandated that the Centers for Disease Control and Prevention (CDC) develop guidelines for FAS diagnosis to be incorporated into standards for medical practice and to be recognized by professional organizations and accrediting boards. These CDC developed guidelines only include diagnostic criteria for FAS and do not incorporate other diagnoses within the FASD continuum62,63. The CDC reported that no existing criteria were uniformly accepted and that the 1996 IOM criteria neither provided reliability and accuracy, nor did the IOM classification take into consideration ethnic or differential diagnostic considerations. As other diagnostic systems have been refined, the CDC guidelines have not been updated.

The CDC consensus identified reports that were used as the scientific basis for creating diagnostic recommendations. With this information, and in coordination with the National Taskforce on Fetal Alcohol Syndrome and Fetal Alcohol Effect (NTFFAS/FAE, another US-funded FAS program), and non-governmental organizations concerned with FAS, CDC formed a scientific working group (SWG) of persons with expertise in research and clinical practice regarding prenatal alcohol exposure to develop diagnostic guidelines for FAS2,64. Guidelines were formulated based on consensus among SWG members and NTFFAS/FAE40,65.

The CDC diagnostic guidelines for FAS (not intended to represent the entire clinical continuum of FASD), published in 2005, are as follows:

Facial dysmorphia: on the basis of racial norms, an affected individual must exhibit all three of the following facial features: smooth philtrum (University of Washington Lip-Philtrum Guide rank 4 or 5); thin vermilion border (University of Washington Lip-Philtrum Guide rank 4 or 5); short palpebral fissures (≤10th centile).

Growth deficiency: prenatal or postnatal height, weight, or both ≤10th centile, adjusted for age, sex, gestational age, and race or ethnicity.

Central nervous system (CNS) abnormalities: structural CNS anomalies (clinically meaningful brain abnormalities observed through imaging); or, head circumference ≤10th centile, adjusted for age and sex.

Neurologic: neurologic problems not resulting from a postnatal insult or fever, or other soft neurologic signs outside normal limits.

Functional: test performance substantially below that expected for an individual’s age, educational level, or circumstances, as evidenced by global cognitive or intellectual deficits representing multiple domains of deficit (or substantial developmental delay in younger children), with performance below the third centile (i.e., 2 SDs below the mean for standardized testing); or functional deficits < 16th centile (i.e., 1 SD below the mean for standardized testing) in at least three of the following domains: cognitive or developmental deficits or discrepancies; executive functioning deficits; motor functioning delays; problems with attention or hyperactivity; problems with social skills; other problems (e.g., sensory problems, pragmatic language problems, memory deficits).

The guidelines were developed to assist service providers in making referral decisions2,17,66. It was recommended that each case be evaluated individually, and that, when in doubt, providers should refer individuals for a full evaluation by a multidisciplinary team with experience in evaluating prenatal alcohol exposure28.

The CDC recommended referral for FASD evaluation in the following circumstances:

When prenatal alcohol exposure is known, a child should be referred for full FAS evaluation when substantial prenatal alcohol use (i.e., seven or more drinks per week, three or more drinks on multiple occasions, or both) has been confirmed. If substantial prenatal alcohol exposure is known, in the absence of any other positive criteria (i.e., dysmorphia, growth deficits, or CNS abnormalities), the primary health-care provider should document this exposure and monitor the child’s ongoing growth and development closely.

When information regarding prenatal alcohol exposure is unknown, a child should be referred for full FAS evaluation for any one of the following:

– A parent or caregiver (foster or adoptive parent) report that a child has or might have FAS;

– All three facial features (i.e., smooth philtrum, thin vermillion border, and short palpebral fissures) are present; 

– One or more facial features are present, in addition to growth deficits in height, weight, or both; one or more facial features are present, and one or more CNS abnormalities are documented; or one or more facial features are present, with growth deficits and one or more CNS abnormalities.

Canadian guideline (2005, updated in 2015)

The Canadian guidelines were first developed in 2005 by a multidisciplinary team comprising Canadian and American experts in the diagnosis of FAS and related disabilities44,67. Review and feedback were provided by a diverse group of individuals, professional organizations and societies, and provincial, territorial and federal levels of government68. The working group endeavored to harmonize existing diagnostic approaches in order to obtain consistent diagnoses of FASD across Canada. The 2005 Canadian guidelines were similar to the Hoyme criteria promulgated in the US, Europe and South Africa, except for the requirement of three (rather than two) sentinel facial features for the diagnosis of FAS and the setting of diagnostic cutoffs at the third (rather than the tenth) centile for growth and neurobehavioral assessments.

The guideline for diagnosis of FASD in Canada underwent significant revision in 2015, resulting in the consolidation of diagnostic categories into two distinct classifications: FASD with sentinel facial features and FASD without sentinel facial features17,69. The simplified diagnostic terminology of the revised Canadian guideline for diagnosis differs markedly from that used in the US and in other international settings, which are based on the original 1996 IOM diagnostic categories. Among other differences in the revised Canadian guideline are the exclusion of the consideration of growth deficiency as a major diagnostic criterion in any diagnostic category and the elimination of ARBD as a diagnostic category, since it is difficult to attribute alcohol as a causative factor of major structural malformations in exposed children, in the absence of other structural or neurobehavioral characteristics of FAS.

Australian guidelines (2016)

In 2013, in response to limited training opportunities in FASD, lack of a nationally adopted diagnostic instrument and confusion about diagnostic criteria, The Australian Guide to the Diagnosis of Fetal Alcohol Spectrum Disorder was developed, and funded by the Commonwealth Department of Health (DoH)47,62. The Australian Guide to Diagnosis is based on and incorporates modifications of the Canadian Guideline.

A diagnosis of FASD requires evidence of prenatal alcohol exposure and severe impairment in three or more domains of central nervous system structure or function. A diagnosis of FASD can be divided into one of two sub-categories37,47,62:

1. FASD with three sentinel facial features (FASD with three sentinel facial features replaces the diagnosis of Fetal Alcohol Syndrome, but without a requirement for growth impairment). The etiological role of alcohol is most clearly established in the presence of all three characteristic facial abnormalities. In this situation a diagnosis of FASD with three sentinel facial features can be made even when prenatal alcohol exposure is unknown, provided there is also severe neurodevelopmental impairment.

2. FASD with less than three sentinel facial features (FASD with less than three sentinel facial features encompasses the previous categories of Partial Fetal Alcohol Syndrome and Neurodevelopmental Disorder-Alcohol Exposed).

Co-existing or alternative diagnoses including genetic conditions (e.g. microdeletions or duplications), effects of other teratogens and prenatal exposures, as well as the effects of postnatal exposures such as early life trauma and brain injury should be considered5,14,43,70.

Scottish Intercollegiate Guidelines Network (2019)

These guidelines were developed by a multidisciplinary team of Scottish experts using a combination of standard Scottish Intercollegiate Guidelines Network (SIGN) methodology. It was based on a systematic review of the evidence and adaptation of the Canadian guideline for the diagnosis of FASD; however, some elements of the Australian guide to the diagnosis of FASD have also been incorporated2,71,72.

The study group created these recommendations, with permission from the Canadian guideline developers, by considering their work and making minor revisions to align the guidance with practice in Scotland. In Scotland, before these new guidelines were drawn up, diagnoses were based on the classification developed by the World Health Organization (ICD-10)29,62.

The only new recommendations added are from the evidence-based review of the literature on screening for alcohol use during pregnancy. These guidelines were first published in 2019 and were revised in 2022.

Although the Canadian diagnostic categories are: FASD with sentinel facial features and FASD without sentinel facial features, the SIGN prefers to apply the terms ‘FASD without sentinel facial features’ as a descriptor rather than a diagnostic term73,74.

An additional diagnostic category of individuals “at risk for neurodevelopmental disorder and FASD, associated with prenatal alcohol exposure” was introduced to describe individuals with confirmed PAE and some indication of neurodevelopmental problems, who did not fit the diagnostic criteria for FASD40.

Guidelines of Interdisciplinary Group of Polish Professionals (2021)

In 2021, Polish physicians noted several limitations in applying existing diagnostic criteria in the diagnosis of FASD29,67. They argued that terms used in the Hoyme diagnostic guidelines, such as ARBD and ARND, are outdated and suggest a cause-and-effect relationship between alcohol exposure and symptoms that was difficult to document in individual cases3,67. In addition, according to the Polish working group, a significant diagnostic delay results from the Hoyme criteria for ARND, since ARND cannot be reliably diagnosed in children under 3 years of age53,75,76.

The Polish working group recommended distinguishing two fundamental diagnostic categories within FASD:

1. FAS;

2. ND-PAE (neurodevelopmental disorder associated with prenatal alcohol exposure).

It was concluded that to fulfill the criterion of neurobehavioral impairment, it is necessary to meet the following three requirements:

the presence of deficits in at least three cognitive areas. In the case of neurological symptoms and signs, deficits in two areas;

the occurrence of abnormalities in at least three areas from the emotional and social sphere, adaptation disorders, or psychopathological symptoms;

significant impact of the identified deficits and symptoms on everyday life activities and school functioning (in the case of people who have completed their education, the data from the interview are to be taken into account).

The working group reiterated that the evaluation of maternal alcohol consumption during pregnancy is critical for the diagnosis of FASD54,56,77,78. The teratogenic effect of alcohol can vary depending on the timing of exposure (the stage of development of the central nervous system, genetics and other individual factors)77,79. There is no single dose of alcohol that is equally dangerous in each case and it is essential to evaluate the level of alcohol consumption during pregnancy on a case-by-case basis32,51,80-82.

Based on previous studies, and taking into account that, in Poland, a standard drink (one dose) is defined as 10 g of pure alcohol, the working group recommended that alcohol exposure criteria for ND-PAE in an affected child be met if the mother had approximately:

≥8 standard doses of alcohol per week for ≥2 weeks of pregnancy or

≥2 episodes of excessive alcohol consumption (consuming ≥4 doses of alcohol on one occasion).

The Polish guidelines are based on those of the 4-digit code for assessing growth and dysmorphia. Therefore, the fundamental problem to be solved in this regard was the selection of appropriate centile grids for the Polish population59,83.

Conclusions

Children who are suspected to have FASD should be referred to an expert team of specialists for rigorous assessment, including assessment of prenatal alcohol exposure, facial dysmorphism and a complete neurobehavioral evaluation. For this reason, it is important to recognize possible FASD-related signs and symptoms early.

In this review we presented a historical and practical comparison among the various existing diagnostic guidelines for FASD, explaining the rationale for their creation and use. Although the proposed guidelines differ significantly from one another, we believe that it cannot be empirically indicated which system is the best. The choice of which diagnostic guideline to use must be based on the diagnostic capabilities of the clinician(s) and the multidisciplinary team that uses them. We reiterate that today in Italy the guidelines proposed by Hoyme and last revised in 2016 are used; however, the other guidelines that we have compared in this discussion also appear to have a scientific rationale. We do believe that although the differences are not always significant between the results of clinical evaluation using various guidelines, it would be appropriate in the future to try to create a universally accepted diagnostic rubric to avoid misdiagnosis and to allow comparisons among populations.

*Interdisciplinary Study Groups: Sapienza Università di Roma, ISTAT - Istituto nazionale di statistica, AIDEFAD - Associazione Italiana Disordini da Esposizione Fetale ad Alcol e/o Droghe, SITAC - Società italiana per il trattamento dell’alcolismo e delle sue complicanze. SIFASD - Società Italiana Sindrome Feto-Alcolica, ISS - Istituto Superiore di Sanità, SIPPS - Società Italiana di Pediatria Preventiva e Sociale, FIMMG-Lazio - Federazione Italiana dei Medici di Medicina Generale Lazio, SIMPeSV - Società Italiana di Medicina di Prevenzione e degli Stili di Vita, CIPe - Confederazione Italiana Pediatri. Adele Minutillo, Alba Crognale, Alberto Chiriatti, Alberto Spalice, Alessandro Mattia, Andrea Liberti, Angelo Selicorni, Antonella Polimeni, Antonio Greco, Arianna Barzacchi, Camilla Di Dio, Duccio Cordelli, Francesca Fanfarillo, Francesca Tarani, Lina Corbi, Luca Cavalcanti, Lucia Leonardi, Lucia Ruggieri, Luigi Memo, Luigi Meucci, Marco Lucarelli, Maria Chiara David, Camilla Perna, Maria Grazia Piccioni, Maria Pia Graziani, Mario Vitali, Marisa Patrizia Messina, Martina Derme, Nunzia La Maida, Sabrina Venditti, Serafino Zangaro, Sergio Terracina, Silvia Francati, Pier Luigi Bartoletti, Simona Pichini, Stefania Bazzo, Stefania Pipitone.

Conflict of interests: the authors have no conflict of interests to declare.

References

1. Bager H, Christensen LP, Husby S, Bjerregaard L. Biomarkers for the detection of prenatal alcohol exposure: a review. Alcohol Clin Exp Res 2017; 41: 251-61.

2. Lange S, Probst C, Gmel G, Rehm J, Burd L, Popova S. Global prevalence of fetal alcohol spectrum disorder among children and youth: a systematic review and meta-analysis. JAMA Pediatr 2017; 171: 948-56.

3. Adebiyi BO, Mukumbang FC, Beytell AM. A guideline for the prevention and management of Fetal Alcohol Spectrum Disorder in South Africa. BMC Health Serv Res 2019; 19: 809.

4. Ferraguti G, Terracina S, Micangeli G, et al. NGF and BDNF in pediatrics syndromes. Neurosci Biobehav Rev 2023; 145: 105015.

5. Micangeli G, Menghi M, Profeta G, et al. The impact of oxidative stress on pediatrics syndromes. Antioxidants 2022; 11: 1983.

6. Ceccanti M, Hamilton D, Coriale G, et al. Spatial learning in men undergoing alcohol detoxification. Physiol Behav 2015; 149: 324-30.

7. Ceccanti M, Coriale G, Hamilton DA, et al. Virtual Morris task responses in individuals in an abstinence phase from alcohol. Can J Physiol Pharmacol 2018; 96: 128-36.

8. Ciafrè S, Ferraguti G, Greco A, et al. Alcohol as an early life stressor: epigenetics, metabolic, neuroendocrine and neurobehavioral implications. Neurosci Biobehav Rev 2020; 118: 654-68.

9. Ciafrè S, Carito V, Ferraguti G, et al. How alcohol drinking affects our genes: an epigenetic point of view. Biochem Cell Biol 2019; 97: 345-56.

10. D’Angelo A, Petrella C, Greco A, et al. Acute alcohol intoxication: a clinical overview. Clin Ter 2022; 173: 280-91.

11. Carito V, Parlapiano G, Rasio D, et al. Fetal alcohol spectrum disorders in pediatrics. FASD and the pediatrician. Biomed Rev 2018; 29: 27.

12. Terracina S, Ferraguti G, Tarani L, et al. Transgenerational abnormalities induced by paternal preconceptual alcohol drinking. Findings from humans and animal models. Curr Neuropharmacol 2021; 19: 1158-73.

13. Yelin R, Schyr RB, Kot H, et al. Ethanol exposure affects gene expression in the embryonic organizer and reduces retinoic acid levels. Dev Biol 2005; 279: 193-204.

14. Denny LA, Coles S, Blitz R. Fetal Alcohol Syndrome and Fetal Alcohol Spectrum Disorders. Am Fam Physician 2017; 96: 515-22.

15. Colom J, Segura-García L, Bastons-Compta A, et al. Prevalence of Fetal Alcohol Spectrum Disorders (FASD) among children adopted from eastern European countries: Russia and Ukraine. Int J Environ Res Public Health 2021; 18: 1-12.

16. Celec P, Jáni P, Smreková L, et al. Effects of anabolic steroids and antioxidant vitamins on ethanol-induced tissue injury. Life Sci 2003; 74: 419-34.

17. Popova S, Dozet D, Shield K, Rehm J, Burd L. Alcohol’s impact on the fetus. Nutrients 2021; 13: 3452.

18. Howell KK, Lynch ME, Platzman KA, Smith GH, Coles CD. Prenatal alcohol exposure and ability, academic achievement, and school functioning in adolescence: a longitudinal follow-up. J Pediatr Psychol 2006; 31: 116-26.

19. Derme M, Piccioni MG, Brunelli R, et al. Oxidative stress in a mother consuming alcohol during pregnancy and in her newborn: a case report. Antioxidants 2023; 12: 1216.

20. Rouquette J. Influence de la toxicomanie alcoolique parental sur le développement physique et psychique des jeunes enfants. Faculté de médecine de Paris, 1957.

21. Lemoine P, Harousseau H, Borteyru J, Menuet J. Les enfants des parents alcoholiques: anomolies observées a propos de 127 cas. Ouest Med 1968; 25: 476-82.

22. Jones KL, Smith DW, Ulleland CN, Streissguth AP. Pattern of malformation in offspring of chronic alcoholic mothers. Lancet 1973; 301: 1267-71.

23. Jones KL, Smith DW. Recognition of the fetal alcohol syndrome in early infancy. Lancet 1973; 302: 999-1001.

24. May PA, Fiorentino D, Phillip Gossage J, et al. Epidemiology of FASD in a province in Italy: prevalence and characteristics of children in a random sample of schools. Alcohol Clin Exp Res 2006; 30: 1562-75.

25. Olson HC. The effects of prenatal alcohol exposure on child development. Infants Young Child 1994; 6: 10-25.

26. Hoyme HE, May PA, Kalberg WO, et al. A practical clinical approach to diagnosis of fetal alcohol spectrum disorders: clarification of the 1996 institute of medicine criteria. Pediatrics 2005; 115: 39-47.

27. Stratton K, Howe C, Battaglia FC. Fetal alcohol syndrome: diagnosis, epidemiology, prevention, and treatment. Washington, DC: National Academies Press, 1996.

28. Farag M. Diagnostic issues affecting the epidemiology of fetal alcohol spectrum disorders. J Popul Ther Clin Pharmacol 2014; 21: e153-8.

29. Cordero JF. A practical clinical approach to diagnosis of fetal alcohol spectrum disorders: clarification of the 1996 Institute of Medicine Criteria. Pediatrics 2005; 115: 1787.

30. Hoyme HE, Kalberg WO, Elliott AJ, et al. Updated clinical guidelines for diagnosing fetal alcohol spectrum disorders. Pediatrics 2016; 138: e20154256-e20154256.

31. Goulden KJ, Loock CA, Chudley AE, et al. Are FASD guidelines: practical and sustainable? CMAJ 2005; 173: 1070-1.

32. Bertrand J, Floyd LR, Weber MK. Guidelines for identifying and referring persons with fetal alcohol syndrome. MMWR Morb Mortal Wkly Rep 2005; 54 (RR-11): 1-14.

33. Hoyme HE, Hoyme DB, Elliott AJ, et al. A South African mixed race lip/philtrum guide for diagnosis of fetal alcohol spectrum disorders. Am J Med Genet A 2015; 167: 752-5.

34. Tarani L, Micangeli G, Rasio D, et al. Clinical and genetic approach to the dysmorphic child. Biomed Rev 2018; 29: 37-46.

35. May PA, Blankenship J, Marais AS, et al. Maternal alcohol consumption producing fetal alcohol spectrum disorders (FASD): quantity, frequency, and timing of drinking. Drug Alcohol Depend 2013; 133: 502-12.

36. May PA, Hasken JM, Hooper SR, et al. Estimating the community prevalence, child traits, and maternal risk factors of fetal alcohol spectrum disorders (FASD) from a random sample of school children. Drug Alcohol Depend 2021; 227: 108918.

37. Watkins RE, Elliott EJ, Mutch RC, et al. Health professionals’ perceptions about the adoption of existing guidelines for the diagnosis of fetal alcohol spectrum disorders in Australia. BMC Pediatr 2012; 12: 69.

38. Davies L, Dunn M, Chersich M, et al. Developmental delay of infants and young children with and without fetal alcohol spectrum disorder in the Northern Cape Province, South Africa. African J Psychiatry 2011; 14: 298-305.

39. May PA, Fiorentino D, Coriale G, et al. Prevalence of children with severe fetal alcohol spectrum disorders in communities near Rome, Italy: new estimated rates are higher than previous estimates. Int J Environ Res Public Health 2011; 8: 2331-51.

40. Kulaga V, Pragst F, Fulga N, Koren G. Hair analysis of fatty acid ethyl esters in the detection of excessive drinking in the context of fetal alcohol spectrum disorders. Ther Drug Monit 2009; 31: 261-6.

41. May PA, De Vries MM, Marais AS, et al. Replication of high fetal alcohol spectrum disorders prevalence rates, child characteristics, and maternal risk factors in a second sample of rural communities in South Africa. Int J Environ Res Public Health 2017; 14: 522.

42. Mughal R, Hill CM, Joyce A, Dimitriou D. Sleep and cognition in children with fetal alcohol spectrum disorders (Fasd) and children with autism spectrum disorders (Asd). Brain Sci 2020; 10: 1-20.

43. Wagner JC, Tergeist M, Kruse B, Sappok T. [Fetal alcohol spectrum disorders in adults]. Nervenarzt 2020; 91: 1069-79.

44. Coles CD, Bandoli G, Kable JA, del Campo M, Suttie M, Chambers CD. Comparison of three systems for the diagnosis of fetal alcohol spectrum disorders in a community sample. Alcohol Clin Exp Res 2023; 47: 370-81.

45. Astley SJ. Validation of the fetal alcohol spectrum disorder (FASD) 4-Digit Diagnostic Code. J Popul Ther Clin Pharmacol 2013; 20: e416-467.

46. Kambeitz C, Klug MG, Greenmyer J, Popova S, Burd L. Association of adverse childhood experiences and neurodevelopmental disorders in people with fetal alcohol spectrum disorders (FASD) and non-FASD controls. BMC Pediatr 2019; 19: 498.

47. Watkins RE, Elliott EJ, Wilkins A, et al. Recommendations from a consensus development workshop on the diagnosis of fetal alcohol spectrum disorders in Australia. BMC Pediatr 2013; 13: 156.

48. Astley SJ, Clarren SK. Diagnosing the full spectrum of fetal alcohol-exposed individuals: Introducing the 4-digit diagnostic code. Alcohol Alcohol 2000; 35: 400-10.

49. Messina MP, Piccioni MG, Petrella C, et al. Advanced midwifery practice: intrapartum ultrasonography to assess fetal head station and comparison with vaginal digital examination. Minerva Obstet Gynecol 2021; 73: 253-60.

50. Dunbar Winsor K. An invisible problem: stigma and FASD diagnosis in the health and justice professions. Adv Dual Diagn 2021; 14: 8-19.

51. Pichini S, Marchei E, Vagnarelli F, et al. Assessment of prenatal exposure to ethanol by meconium analysis: results of an italian multicenter study. Alcohol Clin Exp Res 2012; 36: 417-24.

52. May PA, Baete A, Russo J, et al. Prevalence and characteristics of fetal alcohol spectrum disorders. Pediatrics 2014; 134: 855-66.

53. Goecke TW, Burger P, Fasching PA, et al. Meconium indicators of maternal alcohol abuse during pregnancy and association with patient characteristics. Biomed Res Int 2014; 2014: 702848.

54. Ferraguti G, Merlino L, Battagliese G, et al. Fetus morphology changes by second-trimester ultrasound in pregnant women drinking alcohol. Addict Biol 2020; 25: e12724.

55. Høiseth G, Bernard JP, Stephanson N, et al. Comparison between the urinary alcohol markers EtG, EtS, and GTOL/5-HIAA in a controlled drinking experiment. Alcohol Alcohol 2008; 43: 187-91.

56. Mattia A, Moschella C, David MC, et al. Development and Validation of a GC-EI-MS/MS Method for Ethyl Glucuronide Quantification in Human Hair. Front Chem 2022; 10: 858205.

57. Lange S, Shield K, Koren G, Rehm J, Popova S. A comparison of the prevalence of prenatal alcohol exposure obtained via maternal self-reports versus meconium testing: a systematic literature review and meta-analysis. BMC Pregnancy Childbirth 2014; 14: 127.

58. Koren G, Cohen R. Quantifying fetal alcohol exposure by meconium fatty acid ethyl esters (FAEE); association with adverse fetal outcomes and population estimates of fetal alcohol exposure. Drug Metab Rev 2019; 51: 524-32.

59. Astley SJ, Bledsoe JM, Davies JK, Thorne JC. Comparison of the FASD 4-Digit Code and Hoyme et al. 2016 FASD diagnostic guidelines. Adv Pediatr Res 2017; 4: 13.

60. Ostrea EM, Hernandez JD, Bielawski DM, et al. Fatty acid ethyl esters in meconium: are they biomarkers of fetal alcohol exposure and effect? Alcohol Clin Exp Res 2006; 30: 1152-9.

61. Bjørklund G, Meguid NA, El-Bana MA, et al. Oxidative stress in autism spectrum disorder. Mol Neurobiol 2020; 57: 2314-32.

62. Economic Costs of Fetal Alcohol Specturm Disorder (FASD). J Paediatr Child Health 2018; 54: 7-7.

63. Jacobsen B, Lindemann C, Petzina R, Verthein U. The universal and primary prevention of Foetal Alcohol Spectrum Disorders (FASD): a systematic review. J Prev 2022; 43: 297-316.

64. Aragón AS, Coriale G, Fiorentino D, et al. Neuropsychological characteristics of Italian children with fetal alcohol spectrum disorders. Alcohol Clin Exp Res 2008; 32: 1909-19.

65. Cheng CT, Ostrea EM, Alviedo JNB, Banadera FP, Thomas RL. Fatty acid ethyl esters in meconium: a biomarker of fetal alcohol exposure and effect. Exp Biol Med 2021; 246: 380-6.

66. Bearer CF, Gould S, Emerson R, Kinnunen P, Cook CS. Fetal alcohol syndrome and fatty acid ethyl esters. Pediatr Res 1992; 31: 492-5.

67. Okulicz-Kozaryn K, Maryniak A, Borkowska M, S´migiel R, Dylag KA. Diagnosis of Fetal Alcohol Spectrum Disorders (FASDs): guidelines of interdisciplinary group of polish professionals. Int J Environ Res Public Health 2021; 18: 7526.

68. Dugas EN, Poirier M, Basque D, Bouhamdani N, Lebreton L, Leblanc N. Canadian clinical capacity for fetal alcohol spectrum disorder assessment, diagnosis, disclosure and support to children and adolescents: a cross-sectional study. BMJ Open 2022; 12: e065005.

69. Cook JL, Green CR, Lilley CM, et al.; CFASD Research Network. Fetal alcohol spectrum disorder: a guideline for diagnosis across the lifespan. CMAJ 2016; 188: 191-7.

70. Greenmyer JR, Popova S, Klug MG, Burd L. Fetal alcohol spectrum disorder: a systematic review of the cost of and savings from prevention in the United States and Canada. Addiction 2020; 115: 409-17.

71. Lee E, Bristow J, Arkell R, Murphy C. Beyond ‘the choice to drink’ in a UK guideline on FASD: the precautionary principle, pregnancy surveillance, and the managed woman. Heal Risk Soc 2022; 24: 17-35.

72. Walker D, Fisher C, Sherman A, Wybrecht B, Kyndely K. Fetal alcohol spectrum disorders prevention: an exploratory study of women’s use of, attitudes toward, and knowledge about alcohol. J Am Acad Nurse Pract 2005; 17: 187-193.

73. Davis K, Desrocher M, Moore T. Fetal Alcohol Spectrum Disorder: a review of neurodevelopmental findings and interventions. J Dev Phys Disabil 2011; 23: 143-67.

74. Messina MP, D’Angelo A, Battagliese G, et al. Fetal alcohol spectrum disorders awareness in health professionals: implications for psychiatry. Riv Psichiatr 2020; 55: 79-89.

75. Abernethy C, McCall KE, Cooper G, et al. Determining the pattern and prevalence of alcohol consumption in pregnancy by measuring biomarkers in meconium. Arch Dis Child Fetal Neonatal Ed 2018; 103: F216-20.

76. Jan´czewska I, Wierzba J, Cichon´-Kotek M, Jan´czewska A. Fetal alcohol spectrum disorders - diagnostic difficulties in the neonatal period and new diagnostic approaches. Dev Period Med 2019; 23: 60-6.

77. Ferraguti G, Ciolli P, Carito V, et al. Ethylglucuronide in the urine as a marker of alcohol consumption during pregnancy: comparison with four alcohol screening questionnaires. Toxicol Lett 2017; 275: 49-56.

78. Ceci FM, Fiore M, Agostinelli E, et al. Urinary ethyl glucuronide for the assessment of alcohol consumption during pregnancy: comparison between biochemical data and screening questionnaires. Curr Med Chem 2021; 29: 3125-41.

79. Pichini S, Pellegrini M, Gareri J, et al. Liquid chromatography-tandem mass spectrometry for fatty acid ethyl esters in meconium: assessment of prenatal exposure to alcohol in two European cohorts. J Pharm Biomed Anal 2008; 48: 927-33.

80. Zhang Y, Wang H, Li Y, Peng Y. A review of interventions against fetal alcohol spectrum disorder targeting oxidative stress. Int J Dev Neurosci 2018; 71: 140-5.

81. Floyd RL, Decouflé P, Hungerford DW. Alcohol use prior to pregnancy recognition. Am J Prev Med 1999; 17: 101-7.

82. Inkelis SM, Moore EM, Bischoff-Grethe A, Riley EP. Neurodevelopment in adolescents and adults with fetal alcohol spectrum disorders (FASD): a magnetic resonance region of interest analysis. Brain Res 2020; 1732: 146654.

83. Bortolotti F, Raffaelli R, Di Simone N, et al. CDT reference values for monitoring chronic alcohol abuse in pregnancy. Clin Chim Acta 2020; 507: 156-60.

84. Coles CD, Gailey AR, Mulle JG, Kable JA, Lynch ME, Jones KL. A comparison among 5 methods for the clinical diagnosis of Fetal Alcohol Spectrum Disorders. Alcohol Clin Exp Res 2016; 40: 1000-9.

85. Bower C, Elliott EJ, Zimmet M, et al. Australian guide to the diagnosis of foetal alcohol spectrum disorder: a summary. J Paediatr Child Health 2017; 53: 1021-3.